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电子鼻用于绿脓杆菌及支气管扩张气道细菌分类鉴定研究

来源: Guillermo Suarez-Cuartin, MD, PhD et al  发布日期: 2019-07-08  访问量: 205


支气管扩张中潜在致病微生物(PPM)的气道定植与临床结果相关。电子鼻是一种无创技术,能够区分呼出的呼吸中的挥发性有机化合物(VOC)。目的探讨电子鼻对支气管扩张患者气道细菌定植的鉴别诊断价值。73例临床稳定的支气管扩张患者被纳入研究。用痰液细胞培养法测定PPM的存在。将呼出的气体收集在Tedlar袋中,用Cyranose320电子鼻检测VOC呼吸指纹...
标签: Cyranose 320电子鼻、绿脓杆菌、支气管细菌
 

Identification of Pseudomonas aeruginosa and airway bacterial colonization by an electronic nose in Bronchiectasis

电子鼻用于绿脓杆菌及支气管扩张气道细菌分类鉴定研究

Guillermo Suarez-Cuartin, MD, PhD1,2, Jordi Giner, RN, PhD1,2, José Luis Merino, BSc, PhD3, Ana Rodrigo-Troyano, MD1,2, Anna Feliu, RN1,2, Lidia
Perea, BSc2, Ferran Sanchez-Reus, MD, PhD2,4, Diego Castillo, MD, PhD1,2, Vicente Plaza, MD, PhD1,2, James D. Chalmers, MD, PhD5, Oriol Sibila, MD, PhD1,2.

1Department of Respiratory Medicine, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona. Barcelona, Spain.

2Institut d’Investigació Biomédica Sant Pau (IIB Sant Pau). Barcelona, Spain.

3Electronic Systems Group. Universitat de les Illes Balears. Palma de Mallorca, Spain.

4Department of Microbiology, Hospital de la Santa Creu i Sant Pau. Universitat Autònoma de Barcelona. Barcelona, Spain.

5Scottish Centre for Respiratory Research, University of Dundee. Dundee, UK.


Corresponding author: Dr. Oriol Sibila. Servei de Pneumologia, Hospital de la Santa Creu i Sant Pau. C/. Sant Antoni M. Claret 167, 08025 Barcelona (Spain). E-mail: osibila@santpau.cat


Keywords:
Bronchiectasis; Electronic nose; Pseudomonas aeruginosa; Volatile organic compounds.

关键词:支气管疾病;电子鼻;假单胞菌;挥发性有机化合物。

 

Abstract
Rationale: Airway colonization by Potentially Pathogenic Microorganisms (PPM) in bronchiectasis is associated with worse clinical outcomes. The electronic nose is a non-invasive technology capable of distinguishing volatile organic compounds (VOC) in exhaled breath. We aim to explore if an electronic nose can reliably discriminate airway bacterial colonization in patients with bronchiectasis.
支气管扩张中潜在致病微生物(PPM)的气道定植与临床结果相关。电子鼻是一种无创技术,能够区分呼出的呼吸中的挥发性有机化合物(VOC)。目的探讨电子鼻对支气管扩张患者气道细菌定植的鉴别诊断价值。

Methods: Seventy-three clinically stable bronchiectasis patients were included. PPM presence was determined using sputum culture. Exhaled breath was collected in Tedlar bags and VOC breath-prints were detected by the electronic nose Cyranose 320. Raw data was reduced to three factors with principal component analysis. Univariate ANOVA followed by post-hoc least significant difference test was performed with these factors. Patients were then classified using linear canonical discriminant analysis. Cross-validation accuracy values were defined by the percentage of correctly classified patients.

73例临床稳定的支气管扩张患者被纳入研究。用痰液细胞培养法测定PPM的存在。将呼出的气体收集在Tedlar袋中,用Cyranose320电子鼻检测VOC呼吸指纹。通过主成分分析将原始数据简化为三个因素。对这些因素进行单变量ANOVA分析,然后进行事后最小显著性差异检验。然后采用线性典型判别分析对患者进行分类。交叉验证准确度值由正确分类患者的百分比定义。
Results: Forty-one (56%) patients were colonized with PPM. Pseudomonas aeruginosa (n=27, 66%) and Haemophilus influenzae (n=7, 17%) were the most common PPM. VC breath-prints from colonized and non-colonized patients were significantly different (accuracy of 72%, AUROC 0.75, p<0.001). VOC breath-prints from Pseudomonas aeruginosa colonized patients were significantly different from those of patients colonized with other PPM (accuracy of 89%, AUROC 0.97, p<0.001) and non-colonized patients (accuracy 73%, AUROC 0.83, p=0.007).

41例(56%)患者被PPM定殖。铜绿假单胞菌(n=27,66%)和流感嗜血杆菌(n=7,17%)是最常见的PPM。结肠和非结肠患者的VC呼吸图有显著差异(准确度72%,AUROC 0.75,P<0.001)。铜绿假单胞菌定殖患者的VOC呼吸图谱与其他PPM定殖患者(准确度89%,AUROC 0.97,P<0.001)和非定殖患者(准确度73%,AUROC 0.83,P=0.007)有显著差异。
Conclusions: An electronic nose can accurately identify VOC breath-prints of clinically stable bronchiectasis patients with airway bacterial colonization, especially in those with Pseudomonas aeruginosa.

电子鼻能准确稳定的识别临床支气管扩张患者气道细菌定植的VOC呼吸图谱,尤其是铜绿假单胞菌。


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