tlyonlogo
会员中心
您好,请登录, 注册

最近浏览的商品:

0去询价篮结算
询价篮中还没有商品,赶紧选购吧!
当前位置: 首页 > 实验技术 > 果蔬采后 > 马铃薯块茎对黑心病的易感性受糖和酚类物质的影响
文章分类
相关产品
浏览历史

马铃薯块茎对黑心病的易感性受糖和酚类物质的影响


来源: Elisavet Kiaitsi et al  发布日期: 2021-07-13  访问量: 26


黑心病(BH)是马铃薯块茎的一种生理性疾病,其内部组织在贮藏期间会变色。以前,黑心病的发生与一般的酚类物质积累有关。在这项研究中,在连续两个季节中选择了对BH具有不同敏感性的五个马铃薯种群,在1.5℃或15℃储存一周后,对两个块茎部分(肉和心)中的糖和个别酚类化合物进行了有针对性的分析...
标签: 黑心病、绿原酸异构体、果糖、葡萄糖、气调、保质期
 

Susceptibility to blackheart disorder in potato tubers is influenced by sugar and phenolic profile

马铃薯块茎对黑心病的易感性受糖和酚类物质的影响
Elisavet Kiaitsi, Roberta Tosetti ,Leon A.Terry
Plant Science Laboratory, Cranfield University, Cranfield, MK43 0AL, UK

Highlights
•Cold storage and shelf-life affected potato blackheart development.
•Tuber tissue discoloration enhanced during shelf-life.
•Most susceptible stock accumulated high reducing sugars.
•Chlorogenic acid isomers observed in non-susceptible stocks.
•冷藏和保质期影响马铃薯黑心病的发育。
•在保质期内,块茎组织变色增强。
•最易感的存储积累了高还原糖。
•在非敏感库存中观察到绿原酸异构体。

Abstract

Blackheart (BH) is a physiological disorder of potato tubers in which internal tissue becomes discoloured during storage. The development of BH has been previously linked with general phenolic accumulation. In this study, five potato stocks cv. Maris Piper with different susceptibility to BH were selected across two consecutive seasons, whereupon targeted analysis of sugar and individual phenolic compounds in two tuber sections (flesh and heart) was conducted after storage at 1.5 °C or after one week at 15 °C. The most susceptible stock to BH had the highest accumulation of reducing sugars, while crypto- and neo-chlorogenic acids (chlorogenic acid isomers) were more abundant in flesh tissue of non-susceptible stocks. It is postulated that these metabolites may represent putative pre-symptomatic predictive biomarkers of stock susceptibility to BH.

黑心病(BH)是马铃薯块茎的一种生理性疾病,其内部组织在贮藏期间会变色。以前,黑心病的发生与一般的酚类物质积累有关。在这项研究中,在连续两个季节中选择了对BH具有不同敏感性的五个马铃薯种群,在1.5℃或15℃储存一周后,对两个块茎部分(肉和心)中的糖和个别酚类化合物进行了有针对性的分析。对BH最敏感的种群具有最高的还原糖积累,而非敏感种群的果肉组织中的低温和新绿原酸(绿原酸异构体)更丰富。据推测,这些代谢物可能代表了种群对BH易感性的症状前预测性生物标志物。

2. Materials and methods

2.1. Plant material and experimental design

During two consecutive growing seasons, a total of five stocks of potato (Solanum tuberosum L.) cv. Maris Piper (supplied by Fresh Potato Suppliers Association, FPSA, UK) with different susceptibility to BH were analysed [season 1 (2011–2012), stocks A, B, and C; season 2 (2012–2013), stocks E, and D]. Tuber size ranges between 85−110 mm in length, 68−78 mm in diameter, and 198−270 g in weight. To establish the BH susceptibility of each stock, tubers underwent a “hot-box” procedure developed and carried out at Sutton Bridge Crop Storage Research (SBCSR, UK): unwashed tubers, were initially stored at 3.5 °C and incubated in sealed chambers at 30 °C for 60 h. After incubation, tubers were longitudinally cut in half, placed on trays at room temperature for 24 h, and inspected by SBCSR staff for symptoms of BH (tissue discoloration). Following the hot-box treatment, stocks A, B and D were classified as susceptible, while C and E did not show any discoloration and were ranked as non-susceptible. Tubers of each stock were collected from SBCSR and transported to Cranfield University (CU) across the two consecutive seasons. During each season, tubers were assessed at four time points [0 (baseline, arrival at CU), 8, 16 and 20 weeks] after cold storage (1.5 °C) or after one week under shelf-life conditions (15 °C). At each time point, tubers (9 tubers/stock) were removed from cold storage and assessed as day zero (d0), while another set of tubers (18 and 9 tubers/stock in season 1 and in season 2, respectively) were transferred to 15 °C in air for one week and assessed after seven days (d7) for shelf-life evaluation. Baseline measurements were conducted on tubers of each stock before cold storage (1.5 °C). A total of 108 and 72 tubers per stock were used in season 1 and season 2, respectively. Shelf-life evaluation was conducted in 300 L boxes flushed with air (O2; 21 %, CO2; 0.04 %) controlled by the ICA6000 system (International Controlled Atmosphere Ltd., Paddock Wood, Kent, UK) (Amoah et al., 2017).

在连续两个生长季节中,共分析了5个马铃薯(Solanum tuberosum L.)cv. Maris Piper(由英国新鲜马铃薯供应商协会提供),分析了对BH的不同敏感性[第一季(2011-2012),A、B和C股;第二季(2012-2013),E和D股] 。块茎大小在85-110毫米长,68-78毫米直径,198-270克重量之间。为了确定每个种群的BH易感性,块茎经历了Sutton Bridge Crop Storage Research(SBCSR,英国)开发和实施的 "热箱 "程序:未清洗的块茎,最初储存在3.5℃,在30℃的密封室中培养60小时。在热箱处理后,A、B和D种群被列为易感,而C和E没有显示任何变色,被列为非易感。每个种群的块茎都从SBCSR收集,并在连续两个季节运送到克兰菲尔德大学(CU)。在每个季节,在四个时间点[0(基线,到达CU)、8、16和20周]对冷藏(1.5℃)后或在货架期条件下(15℃)一周后的块茎进行评估。在每个时间点,将块茎(9个块茎/股票)从冷库中取出,作为第0天(d0)进行评估,而另一组块茎(第1季和第2季分别为18和9个块茎/股票)被转移到15℃的空气中一周,7天后(d7)进行货架期评估。在冷库(1.5℃)之前,对每个种群的块茎进行了基线测量。第1季和第2季中,每个种群总共使用了108个和72个块茎。保质期评估是在300L的箱子里进行的,箱子里有空气(O2;21%,CO2;0.04%),由ICA6000气调试验系统(International Controlled Atmosphere Ltd., Paddock Wood, Kent, UK)控制(Amoah等人,2017)。
 


相关文章