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SafePod:表征苹果果实对气调贮藏环境反应的呼吸室


来源: Deborah Rees et al  发布日期: 2021-08-20  访问量: 197


在苹果的长期贮藏过程中,检测低氧压力被用来优化动态气调控制(DCA)贮藏的低氧贮藏制度。监测呼吸特性,特别是呼吸商(RQ),为实现这一目标提供了一个工具。本研究的目的是评估使用呼吸室(SafePod)监测苹果对低氧的反应的协议,该呼吸室设计用于商业气调库和科学研究实验室,并比较RQ反应与来自果皮的叶绿素荧光(CF)产量的变化,这在DCA-CF中使用。...
标签: SafePod、苹果动态气调、呼吸速率、呼吸商
 

SafePod:表征苹果果实对气调贮藏环境反应的呼吸室

SafePod: A respiration chamber to characterise apple fruit response to storage atmospheres

Deborah Rees a,DavidBishopb, James Schaeferc,  RichardColgana,KarenThurstona,RosalindFishera,AndrewDuffb
aNatural Resources Institute, University of Greenwich, Chatham Maritime, Kent, ME4 4TB, UK
bStorage Control Systems Ltd, Bridgehouse, Hop Pocket Lane, Paddock Wood, Kent, TN12 6DQ, UK
cStorage Control Systems Inc, 100 Applewood Drive, Sparta, MI49345, USA

Received 21 March 2021, Revised 18 July 2021, Accepted 25 July 2021, Available online 13 August 2021.

Highlights
•SafePod monitors respiration rate and respiratory quotient of stored fruit.
SafePod监测贮藏水果的呼吸速率和呼吸商
•2–3 day respiratory profile at low O2 allows optimisation of storage conditions.
在低氧条件下,2-3天的呼吸曲线可以优化贮藏条件
•Rise in respiratory quotient at low O2 mirrors chlorophyll fluorescence yield.
低氧条件下呼吸商的上升反映了叶绿素荧光的产量
•Respiratory quotient response is lower as respiration decreases later in storage.
因为呼吸作用在贮藏后期减少,呼吸商的反应较低
•‘Braeburn’ has greater low O2 respiratory stress and alcohol production than ‘Gala’.
Braeburn "比 "Gala "有更大的低氧呼吸压力和酒精产量

 

Abstract

During long-term storage of apples, detection of low O2 stress is used to optimise low O2 storage regimes for dynamic controlled atmosphere (DCA) storage. Monitoring respiratory characteristics, specifically the respiratory quotient (RQ), provides a tool to achieve this. The objectives of this study were to evaluate protocols to monitor response of apple consignments to low O2 using a respiration chamber, the SafePod, designed for use in commercial stores and research laboratories, and to compare the RQ response to changes in chlorophyll fluorescence (CF) yield from the fruit skin as used in DCA-CF.

在苹果的长期贮藏过程中,检测低氧压力被用来优化动态气调控制(DCA)贮藏的低氧贮藏制度。监测呼吸特性,特别是呼吸商(RQ),为实现这一目标提供了一个工具。本研究的目的是评估使用呼吸室(SafePod)监测苹果对低氧的反应的协议,该呼吸室设计用于商业气调库和科学研究实验室,并比较RQ反应与来自果皮的叶绿素荧光(CF)产量的变化,这在DCA-CF中使用。

 

Protocols to identify the lowest oxygen limit (LOL), the O2 concentration below which RQ rises, were tested using ‘Braeburn’ (sensitive to low O2) and ‘Gala’ (less sensitive to low O2). A protocol that allows fruit to acclimatise at each O2 concentration takes several weeks and is therefore not practical for commercial use. A rapid profile without fruit acclimatisation can be completed in 2–3 days. Although this underestimates RQ values, and results in an increase in RQ at a higher O2 concentration than observed for acclimatised fruit, the rapid RQ protocol provides a practical method to compare response of apple consignments between cultivars, orchards and seasons. By the rapid protocol, the LOL of ‘Braeburn’ consignments was near 0.6 kPa and of ‘Gala’ consignments was near 0.2 kPa, consistent with detection of alcoholic taints below the LOL in each case. The RQ response using the SafePod was consistent with increase in CF yield using HarvestWatch™.

使用'Braeburn'(对低氧敏感)和'Gala'(对低氧不太敏感)测试了确定最低氧限(LOL)的协议,即低于RQ上升的O2浓度。让水果在每个O2浓度下适应的方案需要几周的时间,因此对商业用途不实用。不让果实适应的快速分析可以在2-3天内完成。虽然这低估了RQ值,并导致RQ在较高的O2浓度下比适应的水果观察到的要高,但快速RQ协议提供了一个实用的方法来比较不同栽培品种、果园和季节的苹果的反应。通过快速协议,'Braeburn'的LOL接近0.6千帕,'Gala'的LOL接近0.2千帕,与每种情况下检测到的低于LOL的酒精污染相一致。使用SafePod的RQ反应与使用HarvestWatch™的CF产量增加一致。

 

Fruit respiration rates change through the storage season, including a substantial decrease over the first 2 months after harvest. As RQ response is affected by respiration rate, accurate comparison of consignments depends on profiles being measured at the same stage in the storage season. It is more difficult to determine the LOL by RQ profiling later in the season when respiration rates are lower.

果实的呼吸速率在整个贮藏季节都会发生变化,包括在收获后的头两个月内大幅下降。由于RQ反应受呼吸速率的影响,准确比较取决于在储存季节的同一阶段测量的剖面。在贮藏季节后期呼吸速率较低时,通过RQ剖析确定LOL更困难。

 

Keywords:Dynamic controlled atmosphere、Apple (Malus domestica)、Respiratory quotient、Respiration rate、SafePod

关键词:动态气调、苹果(Malus domestica)、呼吸商、呼吸速率、SafePod

 


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