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乙烯、ABA和糖的变化调节黑麦草 低温涝渍下的耐冻性

来源: Barbara Jurczyk et al  发布日期: 2022-01-14  访问量: 12

标签: 乙烯、ABA、糖类

Changes in Ethylene, ABA and Sugars Regulate Freezing Tolerance under Low-Temperature Waterlogging in Lolium perenne


乙烯、ABA和糖的变化调节黑麦草 低温涝渍下的耐冻性

Barbara Jurczyk 1,*,Ewa Pociecha 1,Franciszek Janowiak 2,Michał Dziurka 2,Izabela Kościk 1,Marcin Rapacz 1

1Department of Plant Breeding, Physiology and Seed Science, University of Agriculture in Kraków, Podłużna 3, 30-239 Kraków, Poland
2The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland
Author to whom correspondence should be addressed.
Academic Editor: Tibor Janda
Int. J. Mol. Sci. 2021, 22(13), 6700;
Received: 21 May 2021 / Revised: 16 June 2021 / Accepted: 18 June 2021 / Published: 22 June 2021


Plant overwintering may be affected in the future by climate change. Low-temperature waterlogging, associated with a predicted increase in rainfall during autumn and winter, can affect freezing tolerance, which is the main component of winter hardiness. The aim of this study was to elucidate the mechanism of change in freezing tolerance caused by low-temperature waterlogging in Lolium perenne, a cool-season grass that is well adapted to a cold climate. The work included: (i) a freezing tolerance test (plant regrowth after freezing); (ii) analysis of plant phytohormones production (abscisic acid [ABA] content and ethylene emission); (iii) measurement of leaf water content and stomatal conductance; (iv) carbohydrate analysis; and (v) analysis of Aco1, ABF2, and FT1 transcript accumulation. Freezing tolerance may be improved as a result of cold waterlogging. The mechanism of this change is reliant on multifaceted actions of phytohormones and carbohydrates, whereas ethylene may counteract ABA signaling. The regulation of senescence processes triggered by concerted action of phytohormones and glucose signaling may be an essential component of this mechanism.
Keywords: ABA; cold acclimation; ethylene; freezing tolerance; perennial ryegrass; waterlogging
未来植物越冬可能会受到气候变化的影响。低温涝渍与预计的秋冬季降雨量增加有关,会影响抗冻性,而抗冻性是冬季抗寒性的主要组成部分。本研究的目的是阐明黑麦草低温涝渍引起的耐寒性变化的机制,黑麦草是一种适应寒冷气候的凉季草。工作包括: (i) 耐冻性测试(冷冻后植物再生);(ii) 分析植物激素生产(脱落酸 [ABA] 含量和乙烯排放);(iii) 测量叶片含水量和气孔导度;(iv) 碳水化合物分析;(v) Aco 1、ABF的分析2、FT 1转录本积累。由于冷涝,耐冻性可能会提高。这种变化的机制依赖于植物激素和碳水化合物的多方面作用,而乙烯可能会抵消 ABA 信号传导。由植物激素和葡萄糖信号的协同作用触发的衰老过程的调节可能是该机制的重要组成部分。
关键词: ABA;冷驯化;乙烯;耐冻性;多年生黑麦草;涝

4.3. Ethylene measurement

Ethylene emission was measured in real-time with an electrocatalytic ethylene sensor (EASI-1, Absoger, France). Whole plants without any wounding ET as a result of cutting were individually transferred into closed glass cuvettes (2 dm3). The root system with the soil block was tightly closed in a plastic bag so as not to measure ethylene of soil origin produced by micro-organisms. Measurements were recorded in a ventilated room at ambient temperature in the continuous analysis mode at a time interval of 1 min 10 s and the flow rate of 250 cm3/min. After each measurement, the cuvettes were ventilated. The ethylene sensor was checked before each use, and calibration with standard gas was performed if necessary. After the completion of measurements, the linear regression line was calculated and the derivative function [f′(x)] was calculated to estimate the rate of change of ET emission in both waterlogged and control plants.
4.3. 乙烯测量
使用电催化乙烯传感器(EASI-1,Absoger,France)实时测量乙烯排放。由于切割而没有任何伤害性ET的整株植物被单独转移到封闭的玻璃比色皿(2 dm 3)中。将带有土壤块的根系用塑料袋紧紧封闭,以免测量微生物产生的土壤来源乙烯。在环境温度的通风室内以连续分析模式记录测量值,时间间隔为 1 分 10 秒,流速为 250 cm 3/分钟。每次测量后,将比色皿通风。每次使用前检查乙烯传感器,必要时用标准气体进行校准。测量完成后,计算线性回归线并计算导数函数 [ f '( x )] 以估计淹水和对照植物中 ET 排放的变化率。