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Cyathea delgadii Sternb 的起源.体细胞胚胎由供体组织的发育状态和选定代谢物的相互平衡决定


来源: Anna Mikuła et al  发布日期: 2022-03-08  访问量: 218


体细胞胚胎发生是由配子融合产物以外的细胞形成的植物胚胎。认识到体细胞命运的决定因素的需要促使人们研究供体组织的内源性因素如何决定体细胞胚胎起源的模式。这项研究的开展得益于新开发的树蕨体细胞胚胎发生实验系统。,在无激素的培养基中产生胚胎。比较了产生单细胞或多细胞起源的体细胞胚胎的两种外植体中89种内源化合物(如糖、生长素、细胞分裂素、赤霉素、应激相关激素、酚酸、多胺和氨基酸)的含量和细胞形态学特征...
标签: 氨基酸;碳水化合物;细胞形态学;酚酸;植物激素;多胺;体细胞胚胎发生
 

The Origin of the Cyathea delgadii Sternb. Somatic Embryos Is Determined by the Developmental State of Donor Tissue and Mutual Balance of Selected Metabolites

Cyathea delgadii Sternb 的起源.体体细胞胚胎由供体组织的发育状态和所选代谢物的相互平衡决定

Anna Mikuła 1,*,Wojciech Tomaszewicz 1,Michał Dziurka 2,Andrzej Kaźmierczak 3,Małgorzata Grzyb 1,Mirosław Sobczak 4,Piotr Zdańkowski 5 ,Jan Rybczyński 1

1Center for Biological Diversity Conservation in Powsin—Polish Academy of Sciences Botanical Garden, Prawdziwka 2, 02-973 Warsaw, Poland
2The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland
3Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 141/143, 90-236 Łódź, Poland
4Department of Botany, Institute of Biology, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-787 Warsaw, Poland
5Institute of Micromechanics and Photonics, Warsaw University of Technology, Św. Andrzeja Boboli 8, 02-525 Warsaw, Poland

*Author to whom correspondence should be addressed.
Academic Editor: Alexander E. Kalyuzhny
Cells 2021, 10(6), 1388; https://doi.org/10.3390/cells10061388

Abstract
Somatic embryogenesis is the formation of a plant embryo from a cell other than the product of gametic fusion. The need to recognize the determinants of somatic cell fate has prompted investigations on how endogenous factors of donor tissues can determine the pattern of somatic embryo origin. The undertaking of this study was enabled by the newly developed experimental system of somatic embryogenesis of the tree fern Cyathea delgadii Sternb., in which the embryos are produced in hormone-free medium. The contents of 89 endogenous compounds (such as sugars, auxins, cytokinins, gibberellins, stress-related hormones, phenolic acids, polyamines, and amino acids) and cytomorphological features were compared between two types of explants giving rise to somatic embryos of unicellular or multicellular origin. We found that a large content of maltose, 1-kestose, abscisic acid, biologically active gibberellins, and phenolic acids was characteristic for single-cell somatic embryo formation pattern. In contrast, high levels of starch, callose, kinetin riboside, arginine, and ethylene promoted their multicellular origin. Networks for visualization of the relations between studied compounds were constructed based on the data obtained from analyses of a Pearson correlation coefficient heatmap. Our findings present for the first time detailed features of donor tissue that can play an important role in the somatic-to-embryogenic transition and the somatic embryo origin.

体细胞胚胎发生是由配子融合产物以外的细胞形成的植物胚胎。认识到体细胞命运的决定因素的需要促使人们研究供体组织的内源性因素如何决定体细胞胚胎起源的模式。这项研究的开展得益于新开发的树蕨体细胞胚胎发生实验系统。,在无激素的培养基中产生胚胎。比较了产生单细胞或多细胞起源的体细胞胚胎的两种外植体中89种内源化合物(如糖、生长素、细胞分裂素、赤霉素、应激相关激素、酚酸、多胺和氨基酸)的含量和细胞形态学特征。我们发现,单细胞体细胞胚胎形成模式的特征是含有大量麦芽糖、1-酮糖、脱落酸、生物活性赤霉素和酚酸。相反,高水平的淀粉、胼胝质、激动素核糖体、精氨酸和乙烯促进了它们的多细胞起源。基于皮尔逊相关系数热图分析获得的数据,构建了研究化合物之间关系的可视化网络。我们的研究结果首次展示了供体组织的详细特征,这些特征在体细胞向胚胎转化和体细胞胚胎起源中起着重要作用


Keywords: amino acids; carbohydrates; cytomorphology; phenolic acids; phytohormones; polyamines; somatic embryogenesis

关键词:氨基酸;碳水化合物;细胞形态学;酚酸;植物激素;多胺;体细胞胚胎发生

...

2.3.10. Ethylene
To enable gas sampling from ST and IN samples, 5 explants of each were put into 50 mL Erlenmeyer flasks containing 20 mL of ½MS medium with 1% (w/v) sucrose (see Section 2.1) and kept in the darkness to stabilize cell response to the explant excision. After that, flasks were tightly closed with caps with the inserted pipette tips connected via flexible tubes to a pump of an SCS56 handheld ethylene analyzer (Storage Control System, Sparta, UK). Readings of ethylene production in ppm were taken 1, 4 and 6 h after sampling (Supplementary Figure S2). The ethylene production was stabilized between 4 and 6 h after explant excision. Therefore, the results obtained after 6 h were used for further analyses.

为了能够从ST和IN样本中进行气体取样,将每个样本的5个外植体放入含有20 mL½MS培养基(含1%(w/v)蔗糖)的50 mL锥形烧瓶中(见第2.1节),并保持在黑暗中,以稳定细胞对外植体切除的反应。之后,用盖子将烧瓶紧紧封闭,插入的移液管尖端通过软管连接到SCS56手持乙烯分析(Storage Control System, Sparta, UK)的泵上。取样后1小时、4小时和6小时读取乙烯产量(ppm)(补充图S2)。在外植体切除后4至6小时内,乙烯产量稳定。因此,6小时后获得的结果用于进一步分析。



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