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当前位置: 首页 > 实验技术 > 果蔬采后 > 仙人掌粘液涂层调节细胞壁软化酶,延缓零售条件下储存的香蕉果实的成熟
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仙人掌粘液涂层调节细胞壁软化酶,延缓零售条件下储存的香蕉果实的成熟


来源: Mawande Hugh Shinga et al  发布日期: 2023-12-20  访问量: 63


本研究旨在检验仙人掌粘液(OFIM)可食用涂层在零售条件下最大限度地减少香蕉软化的功效。从刚收获的多刺梨枝条中提取粘液并干燥成粉末。使用液相色谱-质谱法(LC-MS)对OFIM粉末中的酚类化合物进行定量。制备OFIM浓度(1%、2%和3%(w/v)),并检测其物理化学性质。将制备的涂层通过浸渍施加到收获的香蕉果实上,并在室温下储存12天。在实验过程中,测量了几个参数,包括果实重量损失、总可溶性固形物(TSS)、可滴定酸度(TA)、果皮颜色、果肉硬度、乙烯产量、呼吸速率、离子渗漏、丙二醛(MDA)含量、总叶绿素和类胡萝卜素、叶绿素降解酶,果皮中的原果胶含量和水溶性果胶(WSP)及软化相关酶
标签: 细胞破壁、叶绿素降解酶、水果软化
 

Opuntia ficus indica mucilage coatings regulate cell wall softening enzymes and delay the ripening of banana fruit stored at retail conditions

Mawande Hugh Shinga, Olaniyi Amos Fawole

Postharvest and Agroprocessing Research Centre, Department of Botany and Plant Biotechnology, University of Johannesburg, Auckland Park, Johannesburg 2006, South Africa

https://doi.org/10.1016/j.ijbiomac.2023.125550

Highlights
•Efficacy of Opuntia ficus indica mucilage (OFIM) edible coating on bananas was examined.
•OFIM treatments delayed cell wall and chlorophyll degradation in bananas.
•OFIM coating maintained quality and extended shelf life by suppressing softening enzymes.
•Phenolic compounds in OFIM powder were characterized using LC-MS.
•2 % concentration of OFIM coating was found to be the most effective in maintaining banana quality.

Abstract
Rapid ripening and softening due to cell wall polysaccharide degradation and disassembly pose major challenges in extending fruit storability. This study aimed to examine the efficacy of Opuntia ficus indica mucilage (OFIM) edible coating in minimizing softening in bananas under retail conditions. Mucilage was extracted from freshly harvested prickly pear cladodes and dried into a powder. Phenolic compounds in OFIM powder were quantified using liquid chromatography-mass spectrometry (LC-MS). OFIM concentrations (1, 2 and 3 % (w/v)) were prepared, and their physicochemical properties were examined. The prepared coatings were applied to harvested banana fruit by dipping and stored at room temperature for 12 days. During the experiment, several parameters were measured, including fruit weight loss, total soluble solids (TSS), titratable acidity (TA), peel color, pulp firmness, ethylene production, respiration rate, ion leakage, malondialdehyde (MDA) content, total chlorophyll and carotenoids, chlorophyll-degrading enzymes, protopectin content and water-soluble pectin (WSP) and softening-related enzymes in the peel. Results showed that mucilage treatments effectively delayed cell wall and chlorophyll degradation, as well as carotenoid accumulation, thus inhibiting ripening-associated processes compared to control fruit. OFIM-treated fruit exhibited significantly higher firmness, chlorophyll content, and TA, lower TSS content, ethylene production, respiration rate, MDA concentration, ion leakage and protopectin content than uncoated fruit. This suggests that OFIM edible coating has the potential to maintain quality and extend the shelf life of bananas by suppressing softening enzymes during storage.

由于细胞壁多糖的降解和分解而导致的快速成熟和软化对延长果实的贮藏性提出了重大挑战。本研究旨在检验仙人掌粘液(OFIM)可食用涂层在零售条件下最大限度地减少香蕉软化的功效。从刚收获的多刺梨枝条中提取粘液并干燥成粉末。使用液相色谱-质谱法(LC-MS)对OFIM粉末中的酚类化合物进行定量。制备OFIM浓度(1%、2%和3%(w/v)),并检测其物理化学性质。将制备的涂层通过浸渍施加到收获的香蕉果实上,并在室温下储存12天。在实验过程中,测量了几个参数,包括果实重量损失、总可溶性固形物(TSS)、可滴定酸度(TA)、果皮颜色、果肉硬度、乙烯产量、呼吸速率、离子渗漏、丙二醛(MDA)含量、总叶绿素和类胡萝卜素、叶绿素降解酶,果皮中的原果胶含量和水溶性果胶(WSP)及软化相关酶。结果表明,与对照果实相比,粘液处理有效地延缓了细胞壁和叶绿素的降解,以及类胡萝卜素的积累,从而抑制了成熟相关过程。OFIM处理果实的硬度、叶绿素含量和TA显著高于未处理果实,TSS含量、乙烯产量、呼吸速率、MDA浓度、离子渗漏和原果胶含量显著低于未处理果实。这表明OFIM可食用涂层有可能通过抑制储存过程中的软化酶来保持香蕉的质量并延长其保质期。

Keywords:Cell wall disassembly;Chlorophyll degrading enzymes;Fruit softening

···

2.4. Ethylene production and respiration rate
Banana respiration rate was determined using the closed system method as described by Fawole and Opara [49]. Briefly, seven fruit (of known weight) were placed in a 3 L glass jar, hermetically sealed with a lid containing a rubber septum and placed at room temperature (25 °C) for 1 h. Carbon dioxide production inside the glass jar was measured after incubation using an infrared gas analyzer (Checkmate 3, PBI Dansensor, Ringsted, Denmark). The results obtained for respiration rate were expressed as (mL CO2/kg/h). Ethylene production was measured using an SCS56 ethylene analyzer (Storage Control Systems Ltd., Kent, UK). Results obtained for ethylene were expressed as μL C2 H4 /kg/h.

使用Fawole和Opara[49]所述的封闭系统方法测定香蕉的呼吸速率。简言之,将七种水果(已知重量)放入3L玻璃罐中,用含有橡胶隔膜的盖子密封,并在室温(25°C)下放置1小时。培养后,使用红外气体分析(Checkmate 3,PBI Dansensor,Ringsted,Denmark)测量玻璃罐内的二氧化碳生成。获得的呼吸速率的结果表示为(mL CO2/kg/h)。使用SCS56乙烯分析仪(Storage Control Systems Ltd., Kent, UK)测量乙烯产量。乙烯的结果表示为μL C2 H4/kg/h。

 


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