使用 INFOGEST 消化方法通过蛋白质消化率对基于牛奶、植物和昆虫的蛋白质材料进行营养评估

The INFOGEST method is a valuable tool for understanding and monitoring food digestion as an alternative to in vivo assays. However, few studies have compared animal and alternative protein sources in terms of protein quality using the INFOGEST method. This study aimed to evaluate the protein quality of milk-, plant-, and insect-based protein materials by in vitro protein digestibility and in vitro digestible indispensable amino acid score (DIAAS), following the INFOGEST method. Milk-based protein materials had the highest protein digestibility (86.1–90.8%), followed by soy (85.1%) and wheat (82.3%). These materials had significantly higher protein digestibility compared with zein (65.1%), cricket (63.6%), and mealworm (69.5%). Additionally, the mean values of in vitro DIAAS of milk-based protein materials (105.0–137.5) were higher than those of plant- and insect-based protein materials (1.9–91.0). Milk-based protein materials have higher protein quality than plant- and insect-based protein materials by the nutritional evaluation following the INFOGEST digestion method.

 

INFOGEST 方法是了解和监测食物消化的宝贵工具，可作为体内测定的替代方法。然而，很少有研究使用 INFOGEST 方法在蛋白质质量方面比较动物和替代蛋白质来源。本研究旨在按照 INFOGEST 方法，通过体外蛋白质消化率和体外可消化必需氨基酸评分 (DIAAS) 评估牛奶、植物和昆虫蛋白质材料的蛋白质质量。以牛奶为基础的蛋白质材料具有最高的蛋白质消化率 (86.1–90.8%)，其次是大豆 (85.1%) 和小麦 (82.3%)。与玉米蛋白 (65.1%)、蟋蟀 (63.6%) 和粉虫 (69.5%) 相比，这些材料的蛋白质消化率明显更高。此外，基于牛奶的蛋白质材料的体外 DIAAS 平均值 (105.0–137. 5) 高于基于植物和昆虫的蛋白质材料 (1.9–91.0)。根据 INFOGEST 消化方法进行的营养评价，基于牛奶的蛋白质材料比基于植物和昆虫的蛋白质材料具有更高的蛋白质质量。