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植物中的乙烯检测方法

来源: Simona M. Cristescu et al  发布日期: 2019-11-06  访问量: 21


考虑到乙烯的关键发展和生理作用,气态激素仍然是植物生物学家活跃的研究课题。在了解乙烯生物合成途径以及感知和作用机理方面已取得进展。仍然需要回答许多问题,并验证发现。监测天然气生产通常会完成任何乙烯研究主题的描述。因此,强烈地寻求在田间,温室,实验室或存储设施中为各种植物样品寻找合适的乙烯测量方法...
标签: 乙烯、拟南芥、气体采样、气相色谱、电化学传感器、激光探测器
 

 Current methods for detecting ethylene in plants

植物中的乙烯检测方法

Simona M. Cristescu1,*, Julien Mandon1, Denis Arslanov1, Je´roˆme De Pessemier2, Christian Hermans2
and Frans J. M. Harren1
1Department of Molecular and Laser Physics, Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135,
NL 6525 AJ Nijmegen, The Netherlands and 2Laboratory of Plant Physiology and Molecular Genetics, Universite´ Libre de
Bruxelles, Campus Plaine CP 242, Bd du Triomphe, 1050 Brussels, Belgium
* For correspondence. E-mail s.cristescu@science.ru.nl
Received: 29 August 2012 Revision requested: 1 October 2012 Accepted: 30 October 2012 Published electronically: 12 December 2012
 
Background
In view of ethylene's critical developmental and physiological roles the gaseous hormone remains an active research topic for plant biologists. Progress has been made to understand the ethylene biosynthesis pathway and the mechanisms of perception and action. Still numerous questions need to be answered and findings to be validated. Monitoring gas production will very often complete the picture of any ethylene research topic. Therefore the search for suitable ethylene measuring methods for various plant samples either in the field, greenhouses, laboratories or storage facilities is strongly motivated.
考虑到乙烯的关键发展和生理作用,气态激素仍然是植物生物学家活跃的研究课题。在了解乙烯生物合成途径以及感知和作用机理方面已取得进展。仍然需要回答许多问题,并验证发现。监测天然气生产通常会完成任何乙烯研究主题的描述。因此,强烈地寻求在田间,温室,实验室或存储设施中为各种植物样品寻找合适的乙烯测量方法
 
Scope
This review presents an update of the current methods for ethylene monitoring in plants. It focuses on the three most-used methods -gas chromatography detection, electrochemical sensing and optical detection – and compares them in terms of sensitivity, selectivity, time response and price. Guidelines are provided for proper selection and application of the described sensor methodologies and some specific applications are illustrated of laser-based detector for monitoring ethylene given off by Arabidopsis thaliana upon various nutritional treatments.
这篇综述介绍了植物中乙烯监测的最新方法。它着重介绍三种最常用的方法-气相色谱检测,电化学传感和光学检测-并在灵敏度,选择性,时间响应和价格方面进行比较。为正确选择和应用所描述的传感器方法提供了指南,并举例说明了基于激光的检测器的一些特定应用,该检测器用于监测拟南芥在各种营养处理后释放出的乙烯
 
Conclusions
Each method has its advantages and limitations. The choice for the suitable ethylene sensor needs careful consideration and is driven by the requirements for a specific application.
每种方法都有其优点和局限性。合适的乙烯传感器的选择需要仔细考虑,并且受特定应用要求
 
Keywords: Ethylene, Arabidopsis thaliana, gas sampling, gas chromatography, electrochemical sensing, laser-based detector
关键词:乙烯、拟南芥、气体采样、气相色谱、电化学传感器、激光探测器


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