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来源: Pingping Wang et al  发布日期: 2021-04-26  访问量: 181

标签: 果蝇进食,感应神经元,压电传感器,动物代谢

Visceral Mechano-sensing Neurons Control Drosophila Feeding by Using Piezo as a Sensor


Pingping Wang,1 Yinjun Jia,1 Ting Liu,1 Yuh-Nung Jan,2,* and Wei Zhang1,3,*
1School of Life Sciences, Tsinghua-Peking Joint Center for Life Sciences, IDG/McGovern Institute for Brain Research, Tsinghua University,Beijing, 100084, China
2Howard Hughes Medical Institute, Departments of Physiology, Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA
3Lead Contact *Correspondence: (Y.-N.J.), (W.Z.)

Animal feeding is controlled by external sensory cues and internal metabolic states. Does it also depend on enteric neurons that sense mechanical cues to signal fullness of the digestive tract? Here, we identify a group of piezo-expressing neurons innervating the Drosophila crop (the fly equivalent of the stomach) that monitor
crop volume to avoid food overconsumption. These neurons reside in the pars intercerebralis (PI), a neuro-secretory center in the brain involved in homeostatic control, and express insulin-like peptides with well-established roles in regulating food intake and metabolism. Piezo knockdown in these neurons of wild-type flies phenocopies the food overconsumption phenotype of piezo-null mutant flies. Conversely, expression of either fly Piezo or mammalian Piezo1 in these neurons of piezo-null mutants suppresses the overconsumption phenotype. Importantly, Piezo+ neurons at the PI are activated directly by crop distension, thus conveying a rapid satiety signal along the ‘‘brain-gut axis’’ to control feeding.

动物的进食受外部感官提示和内部代谢状态的控制。它是否还依赖于感觉机械信号来提示消化道饱满的肠神经元?在这里,我们确定了一组在果蝇作物(果蝇的腹部蝇)中支配的表达压电的神经元,这些神经元监测作物的体积以避免食物的过度消费。这些神经元驻留在大脑pars(大脑)的神经分泌中心,参与稳态控制,并表达胰岛素样肽,在调节食物的摄取和代谢方面具有公认的作用。这些野生型果蝇神经元中的压电击倒表型是压电食物过量消费的表型 -空突变体果蝇。相反,在空压电体的这些神经元中,无论是飞压电体还是哺乳动物压电体的表达都抑制了过度消费的表型。重要的是,PI处的压电+神经元通过作物膨胀直接激活,从而沿“脑肠轴”传递快速的饱腹感信号来控制进食。

Feeding assay with FlyPAD (Fly Proboscis and Activity Detector)
FlyPAD assay was carried out as previously described (Itskov et al., 2014) with slight modifications. Flies were collected upon eclosion and aged for 4-7 days. For food deprivation, female flies were kept in an empty vial with filter paper soaked with water for 16 hr. Each fly was transferred to one feeding chamber on the FlyPAD board. Feeding was recorded for 20 min. Sips were detected with Python codes (Cap2Sip) translated from the MATLAB codes (Itskov et al., 2014). Cumulative sips duration was calculated as the summation of all sips detected along the 20 min time window.