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来源: Elani Fourie Wiest et al  发布日期: 2021-07-01  访问量: 31

标签: 硫化氢、人体、透皮

Transdermal Detection of Hydrogen Sulfide in Humans

Elani Fourie Wiest, Jacquelyn Brandenburg, Ross Clark, Debra Friedrichsen, Grayson Silaski, Fares Qeadan, Thomas Howdieshell, Reza Shekarriz, Nancy Kanagy

We have developed a novel approach to measure circulating hydrogen sulfide (H2S) non-invasively as a potential way to diagnose and monitor endothelial dysfunction and peripheral artery disease (PAD). PAD is a life-threatening condition caused by arterial constriction and obstruction of blood flow leading to limb ischemia. Current methods to diagnose and monitor PAD lack sensitivity, are expensive, and technically difficult. Recent studies indicate that decreased H2S production is an underlying cause of PAD. Reduced plasma H2S also correlates with endothelial dysfunction in individuals with untreated hypertension, diabetes, sleep apnea, and other cardiovascular diseases. The Transdermal Gas Sensor (TAGS) device was designed to measure transdermal H2S to test the hypothesis that the diffusion rate (and therefore gas phase concentration) of H2S is directly proportional to dermal blood flow in individuals without endothelial disease. Healthy volunteers between the ages of 21–65 were recruited. Exclusion criteria included subjects currently treated for hypertension, hyperlipidemia, and diabetes. Smokers and pregnant women were also excluded. We observed a linear association between TAGS readings and Laser Speckle Contrast Imaging (LSCI), a gold standard measure of skin blood flow, when readings were adjusted for age in the model (r = 0.5717, p = 0.0132, n = 18). Therefore the TAGS device is not inferior to LSCI in measuring skin blood flow in healthy individuals using a non-inferiority margin of 5 (p = 0.0394). The TAGS device has the potential, therefore, to serve as a more sensitive, economical, and easy to use diagnostic tool to use H2S detection as a predictor of dermal blood flow.


我们开发了一种新的方法,无创循环测量硫化氢(H2S),作为诊断和监测内皮功能障碍和外周动脉疾病(PAD)的一种潜在方式。PAD是一种危及生命的疾病,由动脉收缩和血流受阻导致肢体缺血而引起。目前诊断和监测PAD的方法缺乏敏感性,价格昂贵,技术上也很困难。最近的研究表明,H2S的产生减少是PAD的一个基本原因。血浆H2S的减少也与未经治疗的高血压、糖尿病、睡眠呼吸暂停和其他心血管疾病患者的内皮功能紊乱有关。透皮气体传感器(TAGS)装置被设计用来测量透皮H2S,以测试H2S的扩散率(以及因此的气相浓度)与无内皮疾病的人的皮肤血流成正比的假设。招募了年龄在21-65岁之间的健康志愿者。排除标准包括目前接受高血压、高脂血症和糖尿病治疗的对象。吸烟者和怀孕妇女也被排除在外。我们观察到TAGS读数和激光斑点对比成像(LSCI)之间的线性关联,激光斑点对比成像是衡量皮肤血流的金标准,当读数被调整为模型中的年龄(r = 0.5717, p = 0.0132, n = 18)。因此,在测量健康人的皮肤血流方面,TAGS设备并不逊于LSCI,其非劣势幅度为5(P = 0.0394)。因此,TAGS装置有可能作为一种更敏感、更经济、更容易使用的诊断工具,将H2S检测作为皮肤血流的预测指标。